Web4 Spin-column purify your PCR product OR treat it with Cloning Enhancer. Spin-Column Protocol I (p. 9–11) OR Cloning Enhancer Protocol II (p. 11) 5 Set up your In-Fusion … http://www.ebiotrade.com/newsf/2016-12/2016122165726374.htm
BioTechnicalフォーラム [クローニングに困っています]
Web先日別の遺伝子で初めてEnhancerを使用したところ、12時間後にはコロニーが見え始めたので、下手にPCR産物をいじくり回さない方がいいのかもしれないと思い始めました … Webこの方法はTAクローニングとして知られています。. 「TA-Enhancer Cloning Kit」は、Tベクターとライゲーション用試薬を組み合わせたTAクローニング用キットです。. ラ … simon the zealot sawed in half
TOPO克隆技术 Thermo Fisher Scientific - CN
WebTOPO® Cloning. Toposiomerase based cloning, often called TOPO® cloning or TA cloning, is a method that relies on the hybridization of the complementary base pairs adenine (A) and thymine (T). TOPO® cloning utilizes the Taq polymerase which naturally leaves a single adenosine (A) overhang on the 3' end of PCR products. WebJust to add , for me In Fusion with cloning enhancer works better compared to gel purified DNA fragment. Good luck! Cite. 1 Recommendation. You can go through this link: ... Web6021 DNA Ligation Kit, Version 1: 50 Rxns: USD $232.00: Takara's DNA Ligation Kits are simple, two-component systems that allow rapid performance of all cloning procedures requiring the covalent joining of DNA molecules in vitro.Using an optimized buffer system and T4 DNA Ligase, these kits allow ligation in 30 minutes with high transformation … simon the zealot the disciple