High-density oligonucleotide arrays

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Webnucleotide polymorphism array, spectral karyotyping, tiling array Most human cancers are characterized by genetic instabilities. These instabilities manifest themselves as a series of genetic alterations, including discrete mutations and chromosomal aberrations. With the human genome deciphered, high-throughput Web22 de jan. de 2003 · Abstract Motivation: When running experiments that involve multiple high density oligonucleotide arrays, it is important to remove sources of variation …

Light-directed synthesis of high-density oligonucleotide arrays …

http://bmbolstad.com/misc/normalize/bolstad_norm_paper.pdf Web1 de jan. de 1997 · Oligonucleotide arrays are also used for DNA sequencing and genotyping (Gingeras et al., 1998), which is a promising application of the high-density oligonucleotide hybridization platform. ... great southern bank wire transfer

Tiling array - Wikipedia

Web24 de jul. de 2015 · The methods for preparing DNA chips combine photochemistry and photolithography with solid-phase DNA synthesis chemistry to generate a high-density oligonucleotide probe array in situ [6,7,8]. These two-dimensional arrays containing hundreds or thousands of oligonucleotide probes provide a powerful DNA sequence … Web18 de mai. de 2007 · In spotted arrays, complementary DNA or long oligonucleotide probes are printed onto the substrate. The two messenger RNA samples of interest are reverse transcribed into complementary DNA, labelled with a different fluorescent dye (green cyanine 3 or red cyanine 5) and mixed together, before being washed over the slide and … Web26 de nov. de 1996 · High-density arrays of oligonucleotide probes are proving to be powerful new tools for large-scale DNA and RNA sequence analysis. A method for … florence baseball schedule

Functional Genomics: High-Density Oligonucleotide Arrays

Web3 de set. de 2015 · Carlon, T. Heim (2006), Thermodynamics of RNA/DNA hybridization in high-density oligonucleotide microarrays, Physica A 362: ... Astrand M, Speed TP. A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics. 2003;19(2):185–93. Web7 de fev. de 2007 · High-density DNA probe arrays provide a massively parallel approach to nucleic acid sequence analysis that is transforming gene-based biomedical research and diagnostics. Light-directed combinatorial oligonucleotide synthesis has enabled the large-scale production of GeneChip® probe arrays which contain several hundred of thousand … great southern bank w republic rdWeb1 de jan. de 1996 · We propose to construct an automated machine to make custom high-density oligonucleotide arrays cheaply and efficiently. Our strategy is a mechanical … great southern bank variable interest rate

"WebThis work presents a low-cost method to produce compact arrays using microporous materials and reagent jetting, particularly useful for producing identical arrays from pre … " - High-density oligonucleotide arrays

High-density oligonucleotide arrays

High-density oligonucleotide array analysis reveals extensive ...

Web17 de jun. de 2008 · Affymetrix high density oligonucleotide expression arrays are widely used across all fields of biological research for measuring genome-wide gene expression. An important step in processing oligonucleotide microarray data is to produce a single value for the gene expression level of an RNA transcript using one of a growing number … Web26 de nov. de 1996 · High-density arrays of oligonucleotide probes are proving to be powerful new tools for large-scale DNA and RNA sequence analysis. A method for …

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Web1 de jan. de 2002 · High-density oligonucleotide arrays (gene chips): The probe is generated in situ on the surface of the matrix. The leader in these arrays is Affymetrix … Web1 de set. de 2011 · With flexible sample preparation, high-density oligonucleotide arrays can be tailored for even larger scale genome-wide SNP discovery as well as validation. Show less See publication ...

Web19 de set. de 2003 · High-density oligonucleotide arrays (1, 2) have revolutionized the study of gene expression.The technology enables researchers to detect and quantify tens of thousands of transcripts in a single experiment and has become a standard for the discovery of gene functions, drug evaluation, pathway dissection, and classification of clinical … WebAlthough oligonucleotide probes are often used in "spotted" microarrays, the term "oligonucleotide array" most often refers to a specific technique of manufacturing. …

Web24 de mai. de 1994 · These probe arrays, or DNA chips, can then be applied to parallel DNA hybridization analysis, directly yielding sequence information. In a preliminary experiment, a 1.28 x 1.28 cm array of 256 different octanucleotides was produced in 16 chemical reaction cycles, requiring 4 hr to complete. The hybridization pattern of … Web1 de dez. de 1996 · We have developed an approach that is based on hybridization to small, high-density arrays containing tens of thousands of synthetic oligonucleotides. The …

WebFigure 2: Gene expression monitoring with oligonucleotide arrays. a, A single 1.28×1.28 cm array containing probe sets for approximately 40,000 human genes and ESTs. This array contains features ...

WebAbstract: Genomic DNA-based probe selection by using high density oligonucleotide arrays has recently been applied to heterologous species (Xspecies). With the advent of this new approach, researchers are able to study the genome and transcriptome of a non-model florence baskin harrisburg pennsylvaniaWebIn this paper we report exploratory analyses of high-density oligonucleotide array data from the Affymetrix GeneChip system with the objective of improving upon … florence baseball teamWeb1 de jan. de 1996 · Refereed paperHigh-density oligonucleotide arrays. We are attempting to produce high-density arrays of surface-bound oligonucleotides for hybridization experiments. Our approach is to mechanically miniaturize conventional DNA synthesis chemistry. Ink-jet printer heads are used to deliver small drops of reagents to a … florence baxterWeb23 de dez. de 2002 · We used high-density oligonucleotide microarrays to examine the short- and long-term actions of estradiol (E 2) on the transcriptomes from the medial basal hypothalamus and other brain regions of E 2-treated (10 μg) adult female mice. Our results have revealed several unanticipated gene regulations. great southern bowling leagueWebWe have developed an approach in which sequence information is used directly to design high–density, two–dimensional arrays of synthetic oligonucleotides. The GeneChip® probe arrays are made using spatially patterned, light–directed combinatorial chemical synthesis, and contain up to hundreds of thousands of different oligonucleotides on a small glass … florence beliardWebTo do so, high-density DNA microarrays are fabricated on a base-sensitive monomer that allows the DNA to be conveniently cleaved and retrieved after synthesis and deprotection. The fabrication protocol is optimized so as to limit the number of synthetic errors and to that effect, a layer of β-carotene solution is introduced to absorb UV photons that may … florence bbqWeb22 de jan. de 2003 · Motivation: When running experiments that involve multiple high density oligonucleotide arrays, it is important to remove sources of variation between arrays of non-biological origin. Normalization is a process for reducing this variation. great southern brevet