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Nsp4 toxin

Web20 dec. 2011 · Background Nonstructural glycoprotein 4 (NSP4) encoded by rotavirus is the only viral protein currently believed to function as an enterotoxin. NSP4 is synthesized as an intracellular transmembrane glycoprotein and as such is essential for virus assembly. Infection of polarized Caco-2 cells with rotavirus also results in the secretion of … WebNonstructural protein 4 (NSP4) is the entero-toxin of this virus, which plays its role in the villus epithelial cells and causes diarrhea (2). The non-structural protein 4 (NSP4) is a 175-amino-acid enterotoxin with nascent polypeptide MW Iran J Biotech. 2016 June;14(2): e1233 DOI:10.15171/ijb.1233

Rotavirus NSP4: A Multifunctional Viral Enterotoxin

Web31 mrt. 2005 · Rotavirus NSP4: A Multifunctional Viral Enterotoxin Viral Immunology Viral Immunology Vol. 18, No. 1 Special Focus Section: Enteric Viruses Rotavirus NSP4: A Multifunctional Viral Enterotoxin Dr. Judith M. Ball , Deanne M. Mitchell , Thomas F. Gibbons , and Rebecca D. Parr Published Online: 31 Mar 2005 … mart sewing machines https://fourseasonsoflove.com

How do the rotavirus NSP4 and bacterial enterotoxins …

Web1 mrt. 1998 · The NSP4 gene phylogeny showed that all strains clustered into E1 genotype, ... (CF) alleles on cholera toxin (CT)-induced intestinal secretion was examined in the CF mouse model. Web24 jan. 2006 · The castor-oil plant Ricinus communis A-B dimeric toxin B subunit (RTB) was genetically linked at its N-terminus with a 90 amino acid peptide from simian rotavirus SA-11 non-structural protein NSP4(90) and produced in Escherichia coli BL21 cells. Biologically active recombinant NSP4(90)-RTB fusion p … Web20 dec. 2011 · PDF Nonstructural glycoprotein 4 (NSP4) encoded by rotavirus is the only viral protein currently believed to function as an enterotoxin. NSP4 is... Find, read … hungry resto

Rotavirus toxin NSP4 induces diarrhea by activation of

Category:NSP4 (rotavirus) - Wikipedia

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Nsp4 toxin

The Rotavirus NSP4 Viroporin Domain is a Calcium-conducting …

Web3 mrt. 2024 · Rotavirus nonstructural protein 4 (NSP4) is an endoplasmic reticulum transmembrane glycoprotein that has a viroporin domain (VPD), and NSP4 viroporin … Enterotoxins can be formed by the bacterial pathogens Staphylococcus aureus and Bacillus cereus and can cause Staphylococcal Food Poisoning and Bacillus cereus diarrheal disease, respectively. Staphylococcal enterotoxins and streptococcal exotoxins constitute a family of biologically and structurally related pyrogenic superantigens. 25 staphylococcal enterotoxins (SEs), mainly produced by Staphylococcus aureus, have been identified to date and named alp…

Nsp4 toxin

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Web24 jan. 2006 · Biologically active recombinant NSP4(90)-RTB fusion protein was shown to bind glycoprotein asialofetuin receptor molecules in an in vitro enzyme-linked … Web1 okt. 2005 · Oral immunization with a shiga toxin B subunit: rotavirus NSP4(90) fusion protein protects mice against gastroenteritis. Nak-Won Choi Center for Molecular Biology …

WebBoth the full-length (FL-NSP4) and the spliced (S-NSP4) cDNA amplicons were cloned into pET-32c and pGEX-6P-2. Expression levels of the recombinant proteins were evaluated in E. coli BL21 (DE3) by Western blot analysis. In addition, the toxicity of pET plasmids bearing the S-NSP4 and FL-NSP4 fragments was investigated by plasmid stability test. Web22 mrt. 2003 · Arakawa T, Yu J, Langridge WH (2001) Synthesis of a cholera toxin B subunit-rotavirus NSP4 fusion protein in potato. Plant Cell Rep 20:343–348. Article CAS Google Scholar Ball JM, Tian P, Zeng CQ, Morris A, Estes MK (1996) Age-dependent diarrhea is induced by a viral nonstructural glycoprotein. Science 272:101–104

Web25 okt. 2005 · A fusion protein containing the shiga toxin-1 B subunit (STB) linked to a 90 amino acid peptide (aa residues 86--175) from simian rotavirus (SA--11) nonstructural protein NSP4 was synthesized in Escherichia coli. Mice orally inoculated with 60 microg of STB::NSP4(90) fusion protein per dose generate … Web19 nov. 2024 · NSP4 is required for mast cell-mediated vascular leakage in skin and joints. Mast cell activation contributes to the pathogenesis of allergic and autoimmune diseases. …

Web24 jan. 2006 · A novel targeted fusion toxin protein with full biological activity is reported, which reacted poorly with asialofetuin, but bound strongly to IL-2 receptor based on …

WebA transmembrane glycoprotein, NSP4 is organized into three main domains: a three-helical TM domain in the N-terminus (also a viroporin domain), a central cytoplasmic coiled-coil domain for multimerization, and an C-terminal flexible region. It can also be secreted … marts fish and chipsWebNSP4 and the synthetic peptide NSP4 114–135 have been demonstrated to cause diarrhea in rodents aged 10–14 days [3, 27]. Infected intestinal cells release NSP4 into the intestinal lumen, where it acts as a viral toxin inducing Ca2+-dependent Cl− secretion, and inhibition of the Na+/glucose-cotransporter SGLT1 [13, 27]. Thus, glucose hungry riceWeb1 jun. 2003 · T.-G. Kim W. H. R. Langridge +1-909-5581000 ext. 81362 +1-909-5580177 [email protected] Department of Biochemistry and Microbiology and Center for Molecular Biology and Gene Therapy, School of Medicine, Loma Linda University, CA 92350, Loma Linda, USA A CTB-NSP4 175 fusion gene encoding the entire 175-aa … hungry right before bed