Tth ssb
WebJun 1, 2024 · 2024-01-01. In the present paper, we propose a new frequency-shift keying continuous phase modulation (FSK-CPM) scheme having, by essence, the interesting feature of single-sideband ( SSB) spectrum providing a very compact frequency occupation. First, the original principle, inspired from quantum physics (levitons), is presented. WebSingle-Stranded DNA Binding Protein (SSB) 以高亲和力协同结合单链 DNA,其与双链 DNA 的结合度不高。与单链 DNA 结合后,SSB 使螺旋二聚体变得不稳定,因此 DNA 聚合酶可以更容易地接触到底物。SSB 参与 DNA 复制并在体内重组。目前它也已用于在电镜下显示单链 DNA。PCR 可通过将这种结合蛋白添加到反应液中而 ...
Tth ssb
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WebAug 15, 2009 · T. thermophilus SSB (TthSSB)–F255P and wild-type TthSSB proteins were prepared as described previously [8]. The proteins were shown to be 99% pure. The … WebMay 17, 2006 · The wild-type T.thermophilus SSB and its mutant were prepared as described in Materials and Methods, which were named TthSSB and TthSSB-255 protein, respectively. Each step of the purification of Tth SSB was analyzed by 12.5% SDS–PAGE with CBB staining ( Figure 1 ).
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WebDescription. Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does … WebOct 1, 2002 · Single-stranded DNA-binding proteins (SSBs) play essential roles in DNA replication, recombination, and repair in bacteria, archaea, and eukarya.We report here the identification, expression, and purification of the SSB-like proteins of the thermophilic bacteria Thermus thermophilus and T. aquaticus.The nucleotide (nt) sequence revealed …
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WebDec 25, 2013 · NaCl, followed by a buffer wash to remove unbound Tth SSB proteins. The buffer containing the indicated Tth SSB and NaCl concentration was then. injected into … shark giving a thumbs upWebJan 1, 2005 · Abstract. In this paper we report that the inclusion of heat-resistant RecA protein from a thermophilic bacteria, Thermus thermophilus, and its cofactor (ATP) in PCR effectively eliminates non-specific PCR products.The effect of RecA protein, which catalyzes pairing between homologous DNA molecules with great fidelity in genetic recombination, … popular dry goodsWebJun 1, 2005 · Recently, we have reported the identification, expression and purification of the SSB-like proteins of the thermophilic bacteria Thermus thermophilus and T. aquaticus … shark give birth or lay eggsWebSSB is known to bind ssDNA, protecting it from cleavage by endonucleases. 4 Furthermore, SSB has been found to reduce secondary structure of ssDNA, improve PCR specificity, … popular during progressive eraWebThis “step up” feature of the SSB reflects the objective of the Savings Bond programme, which is to facilitate long-term savings and investment. Coupon rates for each issuance … Please note that you will not be able to amend or cancel submitted redemption … shark gives virgin birthWebJan 24, 2013 · The four SSB proteins were divided into two groups based on primary sequence analysis. tth and bl21 were similar to that of E. coli (NCBI LOCUS: 1EYG_D), … shark glass recycling llcWebExcellgen Tth-SSB [EG-171] - Return to List . Next shark glass cage